Transmission of Pneumocystis carinii DNA from a patient with P-carinii pneumonia to immunocompetent contact health care workers

Citation
Sl. Vargas et al., Transmission of Pneumocystis carinii DNA from a patient with P-carinii pneumonia to immunocompetent contact health care workers, J CLIN MICR, 38(4), 2000, pp. 1536-1538
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
0095-1137 → ACNP
Volume
38
Issue
4
Year of publication
2000
Pages
1536 - 1538
Database
ISI
SICI code
0095-1137(200004)38:4<1536:TOPCDF>2.0.ZU;2-7
Abstract
The transmission of Pneumocystis carinii from person to person was studied by detecting P. carinii-specific DNA in prospectively obtained noninvasive deep-nasal-swab samples from a child with a documented P. carinii pneumonia (PCP), his mother, two contact health care workers, and 30 hospital staff members who did not enter the patient's room (controls). Nested-DNA amplifi cation was done by using oligonucleotide primers designed for the gene enco ding the mitochondrial Large subunit rRNA of rat P, carinii (P. carinii f. sp, carinii) that amplifies all forms of P. carinii and internal primers sp ecific for human P. carinii (f. sp, hominis). P. carinii f, sp, hominis DNA was detected in samples from the patient and all of his contacts versus no ne of the 30 hospital staff members, The results, as previously shown in mu rine models of P, carinii pneumonia, document that person-to-person transmi ssion of P. carinii is possible. This observation suggests that immunocompr omised patients not on PCP prophylaxis should not enter the room of a patie nt with PCP, and it also raises the question as to whether healthy contacts can transmit the disease to immunocompromised patients at risk.