Alteration in membrane fluidity of rat liver microsomes and of liposomes by protoporphyrin and its anti-lipidoperoxidative effect

Citation
K. Imai et al., Alteration in membrane fluidity of rat liver microsomes and of liposomes by protoporphyrin and its anti-lipidoperoxidative effect, BIOL PHAR B, 23(4), 2000, pp. 415-419
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
23
Issue
4
Year of publication
2000
Pages
415 - 419
Database
ISI
SICI code
0918-6158(200004)23:4<415:AIMFOR>2.0.ZU;2-T
Abstract
The effect of protoporphyrin (PP) on membrane fluidity was investigated by electron paramagnetic resonance spectroscopy using doxyl stearate spin prob es in relation to the anti-lipidperoxidative effect of PP. PP decreased the membrane fluidity in rat liver microsomes at concentrations above 1 mM and also in phosphatidylcholine (PC)-cholesterol (Cho) (100:8, a molar basis) liposomes, The lipid peroxidation stimulated by Fe2+ and L-ascorbic acid in those membrane preparations was attenuated along with the decrease in memb rane fluidity by PP, Similar results were also found in Cho-rich PC (100:30 to 100) liposomes having less fluidity. These results suggest that the dec rease in the membrane fluidity caused by PP may be involved in the antioxid ative action of PP.