Jm. Leggett et al., Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization, HEREDITY, 84(1), 2000, pp. 46-53
Fluorescence in situ hybridization (FISH) was used to localize two transgen
es (gus and bar), carried on plasmids pACT-1F and pUBA, respectively, on mi
totic metaphase squashes of T1 plants of the cultivated hexaploid oat Avena
sativa L. cotransformed by microprojectile bombardment of embryogenic call
us. Among the eight progeny analysed by FISH in each of two lines, we detec
ted plants null, hetero- and homozygous for the two genes in one line, and
plants null and heterozygous for the two genes in the other line. Our resul
ts demonstrated that in the two independent transformation events, the gus
and bar genes had inserted in the same position relative to each other. In
each transformation event, the insertions occurred on D satellite (SAT) chr
omosomes bearing a C genome translocation.