Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization

Citation
Jm. Leggett et al., Chromosomal localization of cotransformed transgenes in the hexaploid cultivated oat Avena sativa L. using fluorescence in situ hybridization, HEREDITY, 84(1), 2000, pp. 46-53
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,"Molecular Biology & Genetics
Journal title
HEREDITY
ISSN journal
0018-067X → ACNP
Volume
84
Issue
1
Year of publication
2000
Pages
46 - 53
Database
ISI
SICI code
0018-067X(200001)84:1<46:CLOCTI>2.0.ZU;2-W
Abstract
Fluorescence in situ hybridization (FISH) was used to localize two transgen es (gus and bar), carried on plasmids pACT-1F and pUBA, respectively, on mi totic metaphase squashes of T1 plants of the cultivated hexaploid oat Avena sativa L. cotransformed by microprojectile bombardment of embryogenic call us. Among the eight progeny analysed by FISH in each of two lines, we detec ted plants null, hetero- and homozygous for the two genes in one line, and plants null and heterozygous for the two genes in the other line. Our resul ts demonstrated that in the two independent transformation events, the gus and bar genes had inserted in the same position relative to each other. In each transformation event, the insertions occurred on D satellite (SAT) chr omosomes bearing a C genome translocation.