Effect of ethylene on Agrobacterium tumefaciens-mediated gene transfer to melon

Citation
H. Ezura et al., Effect of ethylene on Agrobacterium tumefaciens-mediated gene transfer to melon, PLANT BREED, 119(1), 2000, pp. 75-79
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
PLANT BREEDING
ISSN journal
0179-9541 → ACNP
Volume
119
Issue
1
Year of publication
2000
Pages
75 - 79
Database
ISI
SICI code
0179-9541(200002)119:1<75:EOEOAT>2.0.ZU;2-C
Abstract
The effect of ethylene on gene transfer mediated by an Agrobacterium tumefa ciens harbouring a binary vector with the beta-glucuronidase (uidA) gene wa s investigated in melon, Cucumis melo L. Explants excised from melon cotyle dons produced ethylene, the production of which was increased by the additi on of 1-aminocyclopropane-1-carboxylic acid (ACC, 20 or 200 mu M), and inhi bited by the addition of aminoethoxyvinylglycin (AVG, 10 mu M). Agrobacteri um inoculation of explants increased ethylene production, while application of AVG during inoculation reduced it. After 4 days of co-cultivation with Agrobacterium, gene transfer in the explants was assayed by transient uidA expression. Application of ACC to the co-cultivation medium reduced Agrobac terium-mediated gene transfer to explants and that of AVG increased it. The se results suggest that ethylene affects the A. tumefaciens-mediated gene t ransfer to the explants excised from melon cotyledons, and the efficiency o f Agrobacterium-mediated gene transfer can be improved by inhibiting ethyle ne production from the explants.