In vivo indomethacin treatment causes microglial activation in adult mice

Citation
Mm. Prechel et al., In vivo indomethacin treatment causes microglial activation in adult mice, NEUROCHEM R, 25(3), 2000, pp. 357-362
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROCHEMICAL RESEARCH
ISSN journal
0364-3190 → ACNP
Volume
25
Issue
3
Year of publication
2000
Pages
357 - 362
Database
ISI
SICI code
0364-3190(200003)25:3<357:IVITCM>2.0.ZU;2-R
Abstract
The current study was undertaken to study the role of prostaglandins in reg ulating microglial activation. Mice were treated with indomethacin (2 mu g/ ml) in their drinking water to selectively inhibit cyclooxygenase activity. After 4-8 days, the effect of inhibiting prostaglandin synthesis on microg lial activity was evaluated. This was accomplished by analyzing microglial expression of Mac-1 (C3 complement receptor) as an indicator of activation. Mac-1 expression was assessed by immunohistochemistry of fixed brain cryos ections, and by flow cytometric analysis of immunostained single cell suspe nsions. Both methods demonstrated that compared to age-matched, untreated c ontrols, brains of indomethacin-treated mice had increased levels of Mac-1 expression, suggesting an increase in the state of microglial activation. T hese results demonstrate the importance of prostaglandins in down regulatin g microglial activity, and that inhibition of prostaglandin synthesis with indomethacin may act to increase the reactivity of the brain's immune syste m.