Polymer-supported nucleic acid fragments - Tools for biotechnology and biomedical research

Citation
H. Seliger et al., Polymer-supported nucleic acid fragments - Tools for biotechnology and biomedical research, REACT FUNCT, 43(3), 2000, pp. 325-339
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
REACTIVE & FUNCTIONAL POLYMERS
ISSN journal
1381-5148 → ACNP
Volume
43
Issue
3
Year of publication
2000
Pages
325 - 339
Database
ISI
SICI code
1381-5148(20000315)43:3<325:PNAF-T>2.0.ZU;2-A
Abstract
A survey is given on recent work in the authors' laboratories dealing with the preparation and application of functional polymers, which are to be use d (a) as supports for nucleic acid synthesis and (b) as carriers mediating the interaction of the immobilized nucleic acid chains with dissolved macro molecules. Two systems are spotlighted, which require loading capacities (1 ) as high as possible, or (2) as low as possible. These are (1) libraries o f oligonucleotides bound either to particles or to a polypropylene tape. Ad (2) the possibility of loading a carrier with a single DNA molecule for ex onucleolytic sequence analysis is discussed. The requirements for appropria te functional polymers for both applications are in the focus of this artic le. (C) 2000 Elsevier Science B.V. All rights reserved.