Acetyl-seryl-aspartyl-lysyl-proline is a novel natural cell cycle regulator of renal cells

Citation
N. Iwamoto et al., Acetyl-seryl-aspartyl-lysyl-proline is a novel natural cell cycle regulator of renal cells, LIFE SCI, 66(15), 2000, pp. PL221-PL226
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
LIFE SCIENCES
ISSN journal
0024-3205 → ACNP
Volume
66
Issue
15
Year of publication
2000
Pages
PL221 - PL226
Database
ISI
SICI code
0024-3205(20000303)66:15<PL221:AIANNC>2.0.ZU;2-L
Abstract
A natural tetrapeptide, acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) is a p hysiological negative regulator of hematopoiesis. The precursor of AcSDKP, thymocin (341 is expressed in many tissues including kidney. The present st udy examined the antiproliferative effect of AcSDKP in two renal cell lines , namely, renal interstitial fibloblasts cell line (NRK 49F) and renal prox imal tubular epithelial cells (LLC-PK1). An addition of AcSDKP for 48 hours in theses cells resulted in a concentration-dependent attenuation in the p roliferation rate (significant difference to non-treated cells was observed at 10(-9) to 10(-5) M AcSDKP) determined by a colorimetry of alamer blue o xidation. The cell cycle analysis of NRK 49F cells treated with AcSDKP show ed that AcSDKP significantly reduced the ratio of S-phase to G2/M-phases. T hus, physiological concentrations of AcSDKP is capable of altering cell cyc le to inhibit the proliferation of renal cells. (C) 2000 Elsevier Science I nc.