Exon 5 and spermine regulate deactivation of NMDA receptor subtypes

Citation
G. Rumbaugh et al., Exon 5 and spermine regulate deactivation of NMDA receptor subtypes, J NEUROPHYS, 83(3), 2000, pp. 1300-1306
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROPHYSIOLOGY
ISSN journal
0022-3077 → ACNP
Volume
83
Issue
3
Year of publication
2000
Pages
1300 - 1306
Database
ISI
SICI code
0022-3077(200003)83:3<1300:E5ASRD>2.0.ZU;2-G
Abstract
Deactivation of N-methyl-D-aspartate (NMDA) channels after brief agonist ex posure determines the duration of their synaptic activation during excitato ry neurotransmission. We performed patch-clamp recordings of L-glutamate re sponses from human embryonic kidney tumoral cells (HEK293) expressing NR1 s ubunit variants lacking exon 5 together with the NR2B subunit. These respon ses had deactivation components that lasted several seconds. The presence o f exon 5 or spermine greatly accelerated deactivation of L-glutamate respon ses through alterations in desensitization. These effects were also observe d at positive holding potentials and in the presence of physiological Mg2+. Thus NR1 splicing and polyamines may have profound effects on the kinetics of NMDA receptor-mediated synaptic transmission.