The TATA motif specifies the differential activation of minimal promoters by varicella zoster virus immediate-early regulatory protein IE62

Authors
Citation
Lp. Perera, The TATA motif specifies the differential activation of minimal promoters by varicella zoster virus immediate-early regulatory protein IE62, J BIOL CHEM, 275(1), 2000, pp. 487-496
Citations number
68
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
275
Issue
1
Year of publication
2000
Pages
487 - 496
Database
ISI
SICI code
0021-9258(20000107)275:1<487:TTMSTD>2.0.ZU;2-I
Abstract
The immediate-early IE62 protein of varicella tester virus is an acidic tra nscriptional activator capable of up-regulating many viral and cellular pro moters with varying efficiencies. We demonstrate that, in the context of a minimal promoter, a TATA element is both sufficient and essential for IE62- mediated transcriptional activation. Differential levels of activation by I E62 in this context were conferred by a panel of naturally occurring sequen ce variations within the TATA motif itself. TATA motif-specific, differenti al induction was not obtained when the IE62 acidic activation domain was ta rgeted as a GALE fusion protein to the same panel. The prototype acidic tra nsactivator, VP16 of herpes simplex virus, failed to discriminate between t hese different TATA motifs when they were placed into an appropriate respon sive promoter context. Nonetheless, a chimeric IE62 polypeptide substituted with the VP16 activation domain retained the ability to differentially mod ulate minimal promoters with various TATA motifs. Taken together with its b inding to TATA box-binding protein (TBP) and transcription factor IIB in vi tro, we suggest that IE62 has the unusual ability to achieve differential l evels of transcriptional activation through different TATA motifs, which ma y be accomplished either directly or indirectly by recognizing conformation al variations in DNA-bound TBP, TBP-transcription factor IIA/B, or TBP-TATA -associated factor complexes.