Measurement of 3-nitrotyrosine and 5-nitro-gamma-tocopherol by high-performance liquid chromatography with electrochemical detection

Citation
K. Hensley et al., Measurement of 3-nitrotyrosine and 5-nitro-gamma-tocopherol by high-performance liquid chromatography with electrochemical detection, FREE RAD B, 28(4), 2000, pp. 520-528
Citations number
69
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN journal
0891-5849 → ACNP
Volume
28
Issue
4
Year of publication
2000
Pages
520 - 528
Database
ISI
SICI code
0891-5849(20000215)28:4<520:MO3A5B>2.0.ZU;2-Q
Abstract
Nitric oxide (NO) is a lipophilic gaseous molecule synthesized by the enzym atic oxidation of L-arginine. During periods of inflammation, phagocytic ce lls generate copious quantities of NO and other reactive oxygen species. Th e combination of NO with other reactive oxygen species promotes nitration o f ambient biomolecules, including protein tyrosine residues and membrane-lo calized gamma-tocopherol. The oxidative chemistry of NO and derived redox c ongeners is reviewed. Techniques are described for the determination of 3-n itro-tyrosine and 5-nitro-gamma-tocopherol in biological samples using high -performance liquid chromatography with electrochemical detection. (C) 2000 Elsevier Science Inc.