The Golgi apparatus segregates from the lysosomal/acrosomal vesicle duringrhesus spermiogenesis: Structural alterations

Citation
Rd. Moreno et al., The Golgi apparatus segregates from the lysosomal/acrosomal vesicle duringrhesus spermiogenesis: Structural alterations, DEVELOP BIO, 219(2), 2000, pp. 334-349
Citations number
82
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
DEVELOPMENTAL BIOLOGY
ISSN journal
0012-1606 → ACNP
Volume
219
Issue
2
Year of publication
2000
Pages
334 - 349
Database
ISI
SICI code
0012-1606(20000315)219:2<334:TGASFT>2.0.ZU;2-#
Abstract
The acrosome is an acidic secretory vesicle containing hydrolytic enzymes t hat are involved in the sperm's passage across the zona pellucida. Imaging of the acrosomal vesicle and the Golgi apparatus in live rhesus monkey sper matids was accomplished by using the vital fluorescent probe LysoTracker DN D-26. Concurrently, the dynamics of living spermatid mitochondria was visua lized using the specific probe MitoTracker CMTRos and LysoTracker DND-26 de tected the acrosomal vesicle from its formation through spermatid different iation. LysoTracker DND-26 also labeled the Golgi apparatus in spermatogeni c cells. In spermatocytes the Golgi is spherical and, in round spermatids, it is localized over the acrosomal vesicle, as confirmed by using polyclona l antibodies against Golgin-95/GM130, Golgin-97, and Golgin-160. Using both live LysoTracker DND-26 imaging and Golgi antibodies, we found that the Go lgi apparatus is East off from the acrosomal vesicle and migrates toward th e sperm tail in elongated spermatids. The Golgi is discarded in the cytopla smic droplet and is undetectable in mature ejaculated spermatozoa. The comb ined utilization of three vital fluorescent probes (Hoechst 33342, LysoTrac ker DND-26, and MitoTracker CMTRos) permits the dynamic imaging of four org anelles during primate spermiogenesis: the nucleus, the mitochondria, the a crosomal vesicle, and the Golgi apparatus. (C) 2000 Academic Press.