A simple capillary zone electrophoretic assay for reserpin using end-column amperometric detection at a carbon fiber micro-disk array electrode

Citation
Wr. Jin et al., A simple capillary zone electrophoretic assay for reserpin using end-column amperometric detection at a carbon fiber micro-disk array electrode, ANALYT CHIM, 408(1-2), 2000, pp. 257-262
Citations number
21
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICA CHIMICA ACTA
ISSN journal
0003-2670 → ACNP
Volume
408
Issue
1-2
Year of publication
2000
Pages
257 - 262
Database
ISI
SICI code
0003-2670(20000309)408:1-2<257:ASCZEA>2.0.ZU;2-O
Abstract
Capillary zone electrophoresis was employed for the determination of reserp in using an end-column amperometric detection with a carbon fiber micro-dis k array electrode at a constant potential of +1.30 V vs, saturated calomel electrode. The optimum conditions of separation and detection are 1.0 x 10( -4) mol/l Tris-8.4 x 10(-5) mol/l HCl for the buffer solution, 20 kV for th e separation voltage, 5 kV and 10s for the injection voltage and the inject ion time, respectively. The limit of detection is 8.0 x 10(-7) mol/l or 1.4 fmol (S/N=2). The relative standard deviation is 2.5% for the migration ti me and 1.5% for the electrophoretic peak current. The method was applied to the determination of reserpin in human serum. (C) 2000 Elsevier Science B. V. All rights reserved.