Antisense oligodeoxynucleotide inhibits vascular endothelial growth factorin human glioma cells

Citation
Sx. Zheng et al., Antisense oligodeoxynucleotide inhibits vascular endothelial growth factorin human glioma cells, ACT PHAR SI, 21(3), 2000, pp. 211-214
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
21
Issue
3
Year of publication
2000
Pages
211 - 214
Database
ISI
SICI code
0253-9756(200003)21:3<211:AOIVEG>2.0.ZU;2-4
Abstract
AIM: To study the effects of antisense vascular endothelial growth factor ( VEGF) oligodeoxynucleotide (ODN) on the expression of VEGF in human glioma cell line (A172 cells). METHODS: VEGF mRNA level was measured by semiquanti fication reverse transcriptase polymerase chain reaction (RTPCR). VEGF prot ein expression in the cells was determined by immunohistochemistry. VEGF pr otein level in the media was measured by ELISA. RESULTS: When the cells wer e treated with antisense VEGF ODN (6.25 - 50 mu mol.L-1), VEGF mRNA level i n the cells decreased remarkably in a concentration-dependent manner. No ch ange was found when the cells were treated with sense or missense ODN. When the cells were treated with antisense VEGF ODN 25 mu mol.L-1, VEGF protein level decreased greatly both in the cells and the media. CONCLUSIONS: Anti sense VEGF ODN inhibited VEGF expression specifically in A172 cells in vitr o and thus the results provided the basis for the further studies in vivo.