Lobular distribution pattern of lactate dehydrogenase and 6-phosphogluconate dehydrogenase activity in rat liver

Citation
B. Schmidt et al., Lobular distribution pattern of lactate dehydrogenase and 6-phosphogluconate dehydrogenase activity in rat liver, ACT HISTOCH, 102(1), 2000, pp. 37-47
Citations number
44
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
ACTA HISTOCHEMICA
ISSN journal
0065-1281 → ACNP
Volume
102
Issue
1
Year of publication
2000
Pages
37 - 47
Database
ISI
SICI code
0065-1281(200002)102:1<37:LDPOLD>2.0.ZU;2-G
Abstract
Lactate dehydrogenase (LDH) and 6-phosphogluconate dehydrogenase (6-PGDH) a ctivities were measured in lobular areas expanding between 3 portal tracts and an efferent central vein in the livers of male Wistar rats, using a Low ry technique. The maximum of LDH activity was found in a nearly uniform bro ad area in the lobular periphery. From that area values decreased along per iportal/septal -> perivenous gradients, but only slightly within that area along the periportal -> septal axis of the vascular septum. Maximum values of 6-PGDH activity were present in an intermediate area close to the centra l vein demonstrating a rather inhomogeneous distribution pattern without a clear definition of zonal limits. Our data on the distribution pattern of L DH are in agreement with the concept of the metabolic lobulus and are suppo rted by a recent evaluation of the vascular architecture in rat liver. The lobular distribution pattern of 6-PGDH cannot be interpreted without doubt in accordance with that concept.