Differentiation of verotoxigenic strains of Escherichia coli isolated frompiglets and calves in the Czech Republic

Citation
P. Alexa et al., Differentiation of verotoxigenic strains of Escherichia coli isolated frompiglets and calves in the Czech Republic, VET MED-CZ, 45(2), 2000, pp. 39-43
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
VETERINARNI MEDICINA
ISSN journal
0375-8427 → ACNP
Volume
45
Issue
2
Year of publication
2000
Pages
39 - 43
Database
ISI
SICI code
0375-8427(200002)45:2<39:DOVSOE>2.0.ZU;2-7
Abstract
A set of 451 and 115 strains of Escherichia coli isolated from piglets and calves affected by diarrhoea, respectively, were tested for verotoxin produ ction and type. The production was tested on Vero cells and the type was id entified by PCR. The production of verotoxin was demonstrated in 189 of the porcine strains and 13 of the bovine strains. Most of the porcine verotoxi genic strains belonged to the serogroups O139, O141, O157, and O138. Positi ve for verotoxin production were 83.3% and 78.2% of the strains of the sero groups O139 and O141, respectively. The type VT2e was identified in 184 str ains and a sequence corresponding to VT2c was found in 2 strains. However, the latter yielded negative results in the Vero cells test. Furthermore an unidentified toxin type was demonstrated in three strains positive in the V ero cell culture. The bovine verotoxigenic strains belonged to the serogrou ps O20, O26, O103, O111, and O157. Both VT1 and VT2 were produced by 6 (46. 2%) of the 13 verotoxigenic strains. Only one verotoxin type was produced b y each of the remaining verotoxigenic strains.