Vitreous cryopreservation maintains the function of vascular grafts

Citation
Yc. Song et al., Vitreous cryopreservation maintains the function of vascular grafts, NAT BIOTECH, 18(3), 2000, pp. 296-299
Citations number
31
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
NATURE BIOTECHNOLOGY
ISSN journal
1087-0156 → ACNP
Volume
18
Issue
3
Year of publication
2000
Pages
296 - 299
Database
ISI
SICI code
1087-0156(200003)18:3<296:VCMTFO>2.0.ZU;2-N
Abstract
Avoidance of ice formation during cooling can be achieved by vitrification, which is defined as solidification in an amorphous glassy state that obvia tes ice nucleation and growth. We show that a vitrification approach to sto ring vascular tissue results in markedly improved tissue function compared with a standard method involving freezing. The maximum contractions achieve d in vitrified vessels were >80% of fresh matched controls with similar dru g sensitivities, whereas frozen vessels exhibited maximal contractions belo w 30% of controls and concomitant decreases in drug sensitivity. In vivo st udies of vitrified vessel segments in an autologous transplant model showed no adverse effects of vitreous cryopreservation compared with fresh tissue grafts.