Identification and population structure of Burkholderia stabilis sp nov (formerly Burkholderia cepacia genomovar IV)

Vandamme, P Mahenthiralingam, E Holmes, B Coenye, T Hoste, B De Vos, P Henry, D Speert, DP

P. Vandamme et al., Identification and population structure of Burkholderia stabilis sp nov (formerly Burkholderia cepacia genomovar IV), J CLIN MICR, 38(3), 2000, pp. 1042-1047

26

INGLESE

Article

Clinical Immunolgy & Infectious Disease",Microbiology

JOURNAL OF CLINICAL MICROBIOLOGY

0095-1137 → ACNP

38

3

2000

1042 - 1047

ISI

0095-1137(200003)38:3<1042:IAPSOB>2.0.ZU;2-N

The Burkholderia cepacia complex currently comprises five genomic species, i.e., B. cepacia genomovar I, B. multivorans (formerly known as B. cepacia genomovar II), B. cepacia genomovar III, B. cepacia genomovar IV, and B. vi etnamiensis (also known as B. cepacia genomovar V), In the absence of strai ghtforward diagnostic tests for the identification of B. cepacia genomovars I, III, and IV, the last two genomic species were not formally classified as novel Burkholderia species (genomovar I contains the type strain and the refore retains the name B, cepacia). In the present study, we describe diff erential biochemical tests and a recA gene-based PCR assay for the routine identification of strains currently known as B. cepacia genomovar IV and pr opose formal classification of this organism as Burkholderia stabilis sp, n ov. B, stabilis can indeed be differentiated from all other B. cepacia comp lex strains by the absence of beta-galactosidase activity, from strains of B. cepacia genomovars I and III and B. vietnamiensis by the inability to ox idize sucrose, and from B. multivorans by the lack of growth at 42 degrees C, In addition, analysis with the recA gene-derived primers BCRG41 (5'-ACCG GCGAGCAG GCGCTT-3') and BCRG42 (5'-ACGCCATCGGGCATGGCA-3') specifically allo ws the detection of B. stabilis strains in a conventional PCR assay. Examin ation of a set of 21 B, stabilis strains by means of random amplified polym orphic DNA analysis and pulsed-field gel electrophoresis typing suggested t hat the genome of this organism is highly conserved, which is in sharp cont rast to the generally accepted genomic diversity, variability, and plastici ty among B. cepacia strains.