Quantitative determination of glutathione in single human erythrocytes by capillary zone electrophoresis with electrochemical detection

Jin, WR Li, W Xu, Q

Wr. Jin et al., Quantitative determination of glutathione in single human erythrocytes by capillary zone electrophoresis with electrochemical detection, ELECTROPHOR, 21(4), 2000, pp. 774-779

28

INGLESE

Article

Chemistry & Analysis

ELECTROPHORESIS

0173-0835 → ACNP

21

4

2000

774 - 779

ISI

0173-0835(200003)21:4<774:QDOGIS>2.0.ZU;2-R

Glutathione (GSH) in single human erythrocytes is determined by capillary z one electrophoresis with end-column amperometric detection at a gold/mercur y amalgam microelectrode. A capillary of 10 pm inner diameter is suitable f or determination of GSH in an individual erythrocyte with a good signal-to- noise ratio. The limit of detection is 1 x 10(-7) mol/L or 26 amol and the linear dynamic range is 2 x 10-7 to 2 x 10(-5) mol/L for the capillary. In this method, the calibration line is obtained with a capillary adsorbed bef ore a certain amount of hemoglobin can be used for the quantification of GS H in the external standardization. The whole cell injection and the lack of necessity of a derivatization reaction lead to more accurate and precise r esults, which are closer to the macroscopic values of glutathione in human red blood cell (i.e., hemolysate) than those determined by indirect laser-i nduced fluorescence detection.