Quantitative determination of glutathione in single human erythrocytes by capillary zone electrophoresis with electrochemical detection

Authors
Citation
Wr. Jin et al., Quantitative determination of glutathione in single human erythrocytes by capillary zone electrophoresis with electrochemical detection, ELECTROPHOR, 21(4), 2000, pp. 774-779
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
ELECTROPHORESIS
ISSN journal
0173-0835 → ACNP
Volume
21
Issue
4
Year of publication
2000
Pages
774 - 779
Database
ISI
SICI code
0173-0835(200003)21:4<774:QDOGIS>2.0.ZU;2-R
Abstract
Glutathione (GSH) in single human erythrocytes is determined by capillary z one electrophoresis with end-column amperometric detection at a gold/mercur y amalgam microelectrode. A capillary of 10 pm inner diameter is suitable f or determination of GSH in an individual erythrocyte with a good signal-to- noise ratio. The limit of detection is 1 x 10(-7) mol/L or 26 amol and the linear dynamic range is 2 x 10-7 to 2 x 10(-5) mol/L for the capillary. In this method, the calibration line is obtained with a capillary adsorbed bef ore a certain amount of hemoglobin can be used for the quantification of GS H in the external standardization. The whole cell injection and the lack of necessity of a derivatization reaction lead to more accurate and precise r esults, which are closer to the macroscopic values of glutathione in human red blood cell (i.e., hemolysate) than those determined by indirect laser-i nduced fluorescence detection.