Dendritic cells acquire the MAGE-3 human tumor antigen from apoptotic cells and induce a class I-restricted T cell response

Citation
V. Russo et al., Dendritic cells acquire the MAGE-3 human tumor antigen from apoptotic cells and induce a class I-restricted T cell response, P NAS US, 97(5), 2000, pp. 2185-2190
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
0027-8424 → ACNP
Volume
97
Issue
5
Year of publication
2000
Pages
2185 - 2190
Database
ISI
SICI code
0027-8424(20000229)97:5<2185:DCATMH>2.0.ZU;2-Q
Abstract
In an attempt to transduce monocyte-derived dendritic cells (DCs) with a re troviral vector coding for an intracytoplasmic tumor antigen (TAA), we were confronted by the evident dissociation between the ability of the treated DCs to induce a TAA-specific response, and the presence of integrated vecto r proviral DNA. The TAA, i.e., MAGE-3, was acquired by DCs and presented to immune effecters, thanks to the property of DCs to uptake the apoptotic bo dies released by the irradiated vector-producing cells. Indeed, we observed that upon irradiation vector-producing cells underwent apoptotic cell deat h, monitored by annexin V and propidium iodide staining, and were phagocyto sed by DCs. Lymphocytes obtained from a patient affected by a MAGE-3(+) mel anoma, were stimulated in vitro with autologous DCs previously exposed to i rradiated MAGE-3-expressing cells. This procedure led to the induction of M AGE-3-specific cytotoxic effecters, directed against a yet unknown MAGE-3 e pitope presented by HLA-A*B5201 molecules. These data demonstrate that DCs can present engulfed human TAAs, thus providing strategies for cancer vacci nation.