Conservation of the 17-kilodalton antigen gene within the genus Bartonella

Citation
D. Sweger et al., Conservation of the 17-kilodalton antigen gene within the genus Bartonella, CL DIAG LAB, 7(2), 2000, pp. 251-257
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071-412X → ACNP
Volume
7
Issue
2
Year of publication
2000
Pages
251 - 257
Database
ISI
SICI code
1071-412X(200003)7:2<251:COT1AG>2.0.ZU;2-K
Abstract
The 17-kDa antigen of Bartonella henselae has previously been shown to elic it a strong humoral immune response in patients with cat scratch disease (C SD) and to be useful in screening human serum samples for CSD. In this stud y, PCR amplification of genes homologous to the 17-kDa antigen gene of B. h enselae was performed using genomic DNAs from several species of Bartonella , including the currently recognized human pathogens, Amplicons of similar size were demonstrated using the following chromosomal DNA templates: B. he nselae (two strains), B. quintana (two strains), B. elizabethae, B. clarrid geiae, B, vinsonii subsp. vinsonii, and B. vinsonii subsp. berkhoffii. No e vidence of a B. bacilliformis homolog of the 17-kDa antigen gene was obtain ed using multiple primer pairs. DNA sequencing revealed open reading frames capable of coding for proteins with sizes similar to that of the 17-kDa an tigen of B. henselae in all of the amplicons; however, extensive sequence d ivergence across the genus was noted. Cloning of the amplified products int o pUC19 resulted in recombinants that directed synthesis of homologs of the 17-kDa protein. Immunoblot analysis using human sera from CSD cases demons trated very little cross-reactivity among different species for this protei n. In contrast, immunoblots using rabbit serum raised to the recombinant B. henselae antigen showed extensive cross-reactivity with the proteins of ot her Bartonella species. The data suggest that the use of the 17-kDa antigen as a serologic reagent may allow the development of more specific diagnost ic assays. Furthermore, the nucleotide sequences from the various versions of the 17-kDa antigen gene should be useful for rapid identification of Bar tonella at the species level.