Salmonella pathogenicity island 2-dependent evasion of the phagocyte NADPHoxidase

Citation
A. Vazquez-torres et al., Salmonella pathogenicity island 2-dependent evasion of the phagocyte NADPHoxidase, SCIENCE, 287(5458), 2000, pp. 1655-1658
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary,Multidisciplinary,Multidisciplinary
Journal title
SCIENCE
ISSN journal
0036-8075 → ACNP
Volume
287
Issue
5458
Year of publication
2000
Pages
1655 - 1658
Database
ISI
SICI code
0036-8075(20000303)287:5458<1655:SPI2EO>2.0.ZU;2-K
Abstract
A type III protein secretion system encoded by Salmonella pathogenicity isl and 2 (SPI2) has been found to be required for virulence and survival withi n macrophages. Here, SPI2 was shown to allow Salmonella typhimurium to avoi d NADPH oxidase-dependent killing by macrophages. The ability of SPI2-mutan t bacteria to survive in macrophages and to cause Lethal infection in mice was restored by abrogation of the NADPH oxidase-dependent respiratory burst . Ultrastructural and immunofluorescence microscopy demonstrated efficient Localization of the NADPH oxidase in the proximity of vacuoles containing S PI2-mutant but not wild-type bacteria, suggesting that SPI2 interferes with trafficking of oxidase-containing vesicles to the phagosome.