Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor tau 1 activation domain

Citation
Ae. Wallberg et al., Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor tau 1 activation domain, MOL CELL B, 20(6), 2000, pp. 2004-2013
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR AND CELLULAR BIOLOGY
ISSN journal
0270-7306 → ACNP
Volume
20
Issue
6
Year of publication
2000
Pages
2004 - 2013
Database
ISI
SICI code
0270-7306(200003)20:6<2004:ROTSCR>2.0.ZU;2-W
Abstract
The SWI-SNF complex has been shown to alter nucleosome conformation in an A TP-dependent manner, leading to increased accessibility of nucleosomal DNA to transcription factors. In this study, we show that the SWI-SNF complex c an potentiate the activity of the glucocorticoid receptor (GR) through the N-terminal transactivation domain, tau 1, in both yeast and mammalian cells . GR-sl can directly interact with purified SWI-SNF complex, and mutations in tau 1 that affect the transactivation activity in vivo also directly aff ect tau 1 interaction with SWI-SNF. Furthermore, the SWI-SNF complex can st imulate tau 1-driven transcription from chromatin templates in vitro, Taken together, these results support a model in which the GR can directly recru it the SWI-SNF complex to target promoters during glucocorticoid-dependent gene activation. We also provide evidence that the SWI-SNF and SAGA complex es represent independent pathways of tau 1-mediated activation but play ove rlapping roles that are able to compensate for one another under some condi tions.