Chemiluminescence of adzuki bean and soybean seedlings

Citation
T. Iida et al., Chemiluminescence of adzuki bean and soybean seedlings, LUMINESCENC, 15(1), 2000, pp. 9-13
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
LUMINESCENCE
ISSN journal
1522-7235 → ACNP
Volume
15
Issue
1
Year of publication
2000
Pages
9 - 13
Database
ISI
SICI code
1522-7235(200001/02)15:1<9:COABAS>2.0.ZU;2-S
Abstract
The chemiluminescence of extracts from leguminous seedlings (adzuki bean an d soybean) was investigated. In an H2O2/gallic acid/water extract system, t he photon intensities of adzuki bean seedlings were increased after germina tion and in the hypocotyls ir reached a maximum level during the first 4 da ys of germination. On day 4 after germination, chemiluminescence in the pri mary leaf part exhibited the strongest intensity. Emission spectra showed a main peak at 510 nm, with shoulders at 660 nm, Mechanical injuries to the stems and cotyledons resulted in about a 1.5- and 6.8-fold increase of chem iluminescence, respectively. In an H2O2/70% EtOH extract/HRP system, photon intensities increased after germination and reached a maximum level during the first 2 days of germination, On day 4 after germination, chemiluminesc ence in the root and leaf area was stronger than in any other area. Emissio n spectra showed a main peak at around 570 nm, with shoulders at around 660 nm. The photon intensities of stems and cotyledons after mechanical injuri es resulted in about an 0.72-fold decrease and an 8.8-fold increase in the presence of H2O2 and acetaldehyde (MeCHO), respectively. Copyright (C) 2000 John Wiley & Sons, Ltd.