Determination by capillary zone electrophoresis of berenil, phenamidine, diampron and dibromopropamidine in serum and urine

Citation
B. Rabanal et al., Determination by capillary zone electrophoresis of berenil, phenamidine, diampron and dibromopropamidine in serum and urine, J CHROMAT B, 738(2), 2000, pp. 293-303
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis
Journal title
JOURNAL OF CHROMATOGRAPHY B
ISSN journal
1387-2273 → ACNP
Volume
738
Issue
2
Year of publication
2000
Pages
293 - 303
Database
ISI
SICI code
1387-2273(20000211)738:2<293:DBCZEO>2.0.ZU;2-B
Abstract
A quick, simple and reliable analysis method has been developed in order to determine berenil, phenamidine, diampron and dibromopropamidine by capilla ry zone electrophoresis in samples of serum and urine. In order to define t he operation parameters in CZE, we have carried out a study on how the appa rent electrophoretic mobility (mu(app)) varies when pH, buffer concentratio n, voltage and temperature are modified. Ohm's law plot has been studied, t oo. With the data obtained from this study we have determined the optimum w ork conditions, which are: citrate buffer 25 mM, pH=3.70, 14 kV, 30 degrees C, wavelength of the UV detector: 200 nm, capillary tube: 570 mmx75 mu m. Under these conditions, all the products appear in times between: 7.6 min p henamidine and 8.8 min dibromopropamidine, limits of detection being: beren il: 0.50, phenamidine: 0.25, diampron: 0.40 and dibromopropamidine: 0.80 mu g ml(-1). We have carried out a recovery study with three kinds of extract ion cartridges: Sep-pak C-18 plus, Sep-pak C-8 plus and Oasis HBL for each one of the products in blood and urine. (C) 2000 Elsevier Science B.V. All rights reserved.