Optimization and validation of a method for the determination of caffeine,8-chlorotheophylline and diphenhydramine by isocratic high-performance liquid chromatography - Stress test for stability evaluation

Citation
C. Barbas et al., Optimization and validation of a method for the determination of caffeine,8-chlorotheophylline and diphenhydramine by isocratic high-performance liquid chromatography - Stress test for stability evaluation, J CHROMAT A, 870(1-2), 2000, pp. 97-103
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
870
Issue
1-2
Year of publication
2000
Pages
97 - 103
Database
ISI
SICI code
0021-9673(20000218)870:1-2<97:OAVOAM>2.0.ZU;2-U
Abstract
The optimization of a HPLC method for caffeine, 8-chlorotheophylline and di phenhydramine separation with UV detection at 229 nm is described. The cond itions studied included: stationary phase, compositions of mobile phases wi th pH modulators. Optimal conditions were: SymmetryShield RP8 column and ac etonitrile-(0.01 M H3PO4-triethylamine, pH 2.8) (22:78, v/v). Validation wa s performed using standards and a pharmaceutical preparation containing the compounds described above. Results from both standards and samples show su itable validation parameters. The pharmaceutical grade substances were test ed by factors that could influence the chemical stability. These reaction m ixtures were analyzed to evaluate the capability of the method to separate degradation products. Degradation products did not interfere with the deter mination of the substances tested by the assay. (C) 2000 Elsevier Science B .V. All rights reserved.