Overexpression of heme oxygenase in neuronal cells, the possible interaction with tau

Citation
A. Takeda et al., Overexpression of heme oxygenase in neuronal cells, the possible interaction with tau, J BIOL CHEM, 275(8), 2000, pp. 5395-5399
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
0021-9258 → ACNP
Volume
275
Issue
8
Year of publication
2000
Pages
5395 - 5399
Database
ISI
SICI code
0021-9258(20000225)275:8<5395:OOHOIN>2.0.ZU;2-C
Abstract
Increased expression of heme oxygenase-1 (HO-1) is a common feature in a nu mber of neurodegenerative diseases. Interestingly, the spatial distribution of HO-1 expression in diseased brain is essentially identical to that of p athological expression of tau. In this study, we explored the relationship between HO-1 and tau, using neuroblastoma cells stably transfected with sen se and antisense HO-1 constructs as well as with the vector alone. In trans fected cells overexpressing HO-1, the activity of heme oxygenase was increa sed, and conversely, the level of tau protein was dramatically decreased wh en compared with antisense HO-1 or CEP transfected cells. The suppression o f tau protein expression was almost completely reversed by zinc-deuteroporp hyrin, a specific inhibitor of heme oxygenase activity. The activated forms of ERKs (extracellular signal-regulated kinases) were also decreased in ce lls overexpressing HO-I although no changes in the expression of total ERB- 1/2 proteins were observed. These data are in agreement with the finding th at the expression of tau is regulated through signal cascades including the ERKs, whose activities are modulated by oxidative stresses. The expression of tan and HO-1 may be regulated by oxidative stresses in a coordinated ma nner and play a pivotal role in the cytoprotection of neuronal cells.