Extraction and quantification of benzo[a]pyrene in soil by reversed phase thin layer chromatography

Citation
Dr. Nemergut et al., Extraction and quantification of benzo[a]pyrene in soil by reversed phase thin layer chromatography, J LIQ CHR R, 23(4), 2000, pp. 579-586
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
ISSN journal
1082-6076 → ACNP
Volume
23
Issue
4
Year of publication
2000
Pages
579 - 586
Database
ISI
SICI code
1082-6076(2000)23:4<579:EAQOBI>2.0.ZU;2-7
Abstract
As research on the biodegradation of benzo[a]pyrene (B[a]P) shifts from lab oratory to field based studies, a need has arisen for reproducible, economi cal, and accurate methods for extraction and quantification of B[a]P. This study describes a method for the analysis of B[a]P using batch extraction t echniques with quantification by reversed phase high performance thin layer chromatography (HPTLC) and densitometry. A loam soil was fortified with B[ a]P at a level of 100 mg B[a]P kg(-1) soil. Several solvents were evaluated as potential soil extraction systems including: acetone, acetonitrile, eth yl acetate, methanol, and methylene chloride:acetone (1:1). Reverse phase t hin layer chromatography of B[a]P was performed on hydrocarbon impregnated, C18 reverse phase plates using methanol:acetonitrile (1:1) as the developi ng solvent. The Rf for B[a]P in this system was found to be 0.52. Recovery of B[a]P from soil ranged from 11% with methanol to 84% with ethyl acetate.