Evaluation of different PCR-based DNA fingerprinting techniques for assessing the genetic variability of isolates of the fungus Epicoccum nigrum

Citation
F. Arenal et al., Evaluation of different PCR-based DNA fingerprinting techniques for assessing the genetic variability of isolates of the fungus Epicoccum nigrum, J APPL MICR, 87(6), 1999, pp. 898-906
Citations number
41
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF APPLIED MICROBIOLOGY
ISSN journal
1364-5072 → ACNP
Volume
87
Issue
6
Year of publication
1999
Pages
898 - 906
Database
ISI
SICI code
1364-5072(199912)87:6<898:EODPDF>2.0.ZU;2-N
Abstract
Thirty-six strains of the fungus Epicoccum nigrum, isolated from different substrata and ecosystems of Europe, America and Africa, were analysed using 1+ molecular markers included in 5 different genetic fingerprinting techni ques: AP-PCR, tDNA-PCR, microsatellite-primed PCR, ARDRA and AFLP. All of t he techniques used were able to differentiate the isolates, showing a high genetic diversity within the species. However, the different techniques det ected different levels of similarity among the strains; ARDRA shows the mos t homogeneous results whilst AP-PCR shows the must heterogeneous. The simil arity indices achieved for each strain were compared for the different tech niques. The distribution obtained by microsatellite-primed PCR was similar to those shown bq; AP-PCR techniques. tDNA-PCR and AFLP rendered similar di stributions, and ARDRA showed remarkably different results from the other t echniques. The results also reveal the lad; of an overall correlation betwe en geographical or ecological origin of the isolates and their genotypes.