Differential expression of dihydropyrimidinase-related protein genes in developing and adult enteric nervous system

Citation
H. Inagaki et al., Differential expression of dihydropyrimidinase-related protein genes in developing and adult enteric nervous system, HISTOCHEM C, 113(1), 2000, pp. 37-41
Citations number
18
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN journal
0948-6143 → ACNP
Volume
113
Issue
1
Year of publication
2000
Pages
37 - 41
Database
ISI
SICI code
0948-6143(200001)113:1<37:DEODPG>2.0.ZU;2-1
Abstract
Dihydropyrimidinase-related proteins (DRPs) are involved in axonal outgrowt h and pathfinding. However, little is known about their significance in the enteric nervous system (ENS), the largest and most complex division of the peripheral nervous system. Using in situ hybridization (ISH) and northern blotting, we examined mRNA expression of DRP-1-4 transcripts in the develop ing and adult mouse digestive tract and in the adult human colon. ISH detec ted the mouse DRP-3 transcript in the developing ENS on embryonic day (E)12 and at the later stages as well as in the adult intestine. Mouse DRP-1 and -2 transcripts appeared at E14. DRP-2 transcript was also detected in the adult intestine although DRP-1 expression was lower in the adult. DRP-4 gen e was not expressed in the ENS during development or adulthood whereas the signal was apparent in the developing and adult central nervous system (CNS ). The DRP expression pattern in the human colon was similar to that of the mouse large intestine. Northern blot analysis showed that DRPs were differ entially expressed in the mouse and human intestines, supporting the result s of ISH. These data suggest that DRPs play a role not only in the CNS but also in the ENS.