Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes

Citation
Sx. Zheng et al., Bilobalide promotes expression of glial cell line-derived neurotrophic factor and vascular endothelial growth factor in rat astrocytes, ACT PHAR SI, 21(2), 2000, pp. 151-155
Citations number
12
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
21
Issue
2
Year of publication
2000
Pages
151 - 155
Database
ISI
SICI code
0253-9756(200002)21:2<151:BPEOGC>2.0.ZU;2-S
Abstract
AIM: To study the effects of bilobalide on the expression of glial cell lin e-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF) in rat astrocytes in vitro. METHODS: Semiquantification polymerase chain reaction (SQ-PCR) was used to investigate GDNF and VEGF mRNA expressi on in the astrocytes after bilobalide (5, 15, 50, 100 mu mol.L-1) treatment . Immunohistochemistry method was used to detect GDNF and VEGF protein expr ession in cells treated with bilobalide 50 mu mol.L-1 for 24 h. RESULTS: GD NF and VEGF mRNA increased markedly after astrocytes were treated With bilo balide 50 mu mol.L-1 for 12 h. GDNF and VEGF protein were detected in the c ytoplasm of astrocytes after the cells were treated with bilobalide 50 mu m ol.L-1 for 24 h. CONCLUSION: Bilobalide induced GDNF and VEGF expression in the cultured astrocytes.