Determination of granulocyte-specific antigens on neutrophil Fc gamma receptor IIIb by PCR-preferential homoduplex formation assay, and gene frequencies in the Japanese population

Citation
K. Fujiwara et al., Determination of granulocyte-specific antigens on neutrophil Fc gamma receptor IIIb by PCR-preferential homoduplex formation assay, and gene frequencies in the Japanese population, VOX SANGUIN, 77(4), 1999, pp. 218-222
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
VOX SANGUINIS
ISSN journal
0042-9007 → ACNP
Volume
77
Issue
4
Year of publication
1999
Pages
218 - 222
Database
ISI
SICI code
0042-9007(1999)77:4<218:DOGAON>2.0.ZU;2-L
Abstract
Objectives: Granulocyte-specific antigens play an important role in provoki ng immune neutropenia and transfusion reactions. We developed a new DNA-typ ing method, PCR-preferential homoduplex formation assay (PHFA), to determin e granulocyte-specific antigens on the neutrophil Fc gamma receptor lllb (F cyRlllb, CD16b), namely the NA1, NA2, and SH antigens and their gene freque ncies in the Japanese population. materials and Methods: Four hundred unrel ated healthy Japanese blood donors were typed using PCR-PHFA. To confirm th e accuracy of the results of Fc gamma RlllB genotyping using PCR-PHFA, PCR- sequence-specific primer(SSP) typing and PCR-restriction fragment length po lymorphism (RFLP) typing were carried out in another 20 samples for compari son. Results: The results of PCR-PHFA typing agreed well with other methods . The frequencies of the Fc gamma RlllB alleles were 62.2, 37.8, 0 and 0% f or NA1, NA2, SH, and 'NA-null', respectively. Conclusion: The PCR-PHFA meth od can be semi-automated easily with computer-based assignment and is suita ble for typing both small and large numbers of samples. In the Japanese pop ulation, the frequency of NA1 is about double that in Caucasians (32.5%), a nd the SH allele is rare. Copyright (C) 1999 S. Karger AG. Basel.