Cryopreservation reduces the ability of hamster 2-cell embryos to regulateintracellular pH

Citation
M. Lane et al., Cryopreservation reduces the ability of hamster 2-cell embryos to regulateintracellular pH, HUM REPR, 15(2), 2000, pp. 389-394
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Reproductive Medicine","da verificare
Journal title
HUMAN REPRODUCTION
ISSN journal
0268-1161 → ACNP
Volume
15
Issue
2
Year of publication
2000
Pages
389 - 394
Database
ISI
SICI code
0268-1161(200002)15:2<389:CRTAOH>2.0.ZU;2-L
Abstract
Vitrification of hamster 2-cell embryos impairs the activity of both the Na +/H+ antiporter and HCO3-/Cl- exchanger; the two transport proteins respons ible for the regulation of intracellular pH (pHi). The activities of both t he Na+/H+ antiporter and HCO3-/Cl- exchanger were significantly reduced at 4 h following warming compared to freshly collected embryos. Normal levels of activity of both transporters were not restored until 6 h after warming. Thus, cryopreservation of cleavage stage hamster embryos has a detrimental effect on their ability to maintain intracellular ionic homeostasis. Impai rment of these pHi regulatory proteins resulted in the pHi of embryos being significantly elevated from the control values of 1.2 to 7.35 for approxim ately 4 h after warming. In addition, an elevated pHi value significantly i mpaired oxidative metabolism. Therefore, the loss in developmental competen ce of embryos following cryopreservation mag in part be explained by a redu ced ability to regulate intracellular pH that results in perturbations in m etabolism and disruption of energy production.