Latent hepatitis B virus infection in healthy individuals with antibodies to hepatitis B core antigen

Citation
H. Marusawa et al., Latent hepatitis B virus infection in healthy individuals with antibodies to hepatitis B core antigen, HEPATOLOGY, 31(2), 2000, pp. 488-495
Citations number
45
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Gastroenerology and Hepatology","da verificare
Journal title
HEPATOLOGY
ISSN journal
0270-9139 → ACNP
Volume
31
Issue
2
Year of publication
2000
Pages
488 - 495
Database
ISI
SICI code
0270-9139(200002)31:2<488:LHBVII>2.0.ZU;2-Z
Abstract
Several recent reports have shown that hepatitis B virus (HBV) could be fre quently transmitted to the recipients from donors who have antibodies to he patitis B core antigen (anti-HBc) through liver transplantation. We provide here the molecular evidence of latent HBV infection accompanied with ongoi ng viral replication in the liver tissue of anti-HBc-positive healthy indiv iduals. HBV DNA was detectable in 13 of 14 healthy donors who were positive for both anti-HBc and antibodies to hepatitis B surface antigen (anti-HBs) , but in none of 3 who were positive for anti-HBs alone. The detected HBV g enomes from these subjects included covalently closed circular DNA and preg enomic RNA, the replication intermediate of HBV. Notably, 5 of 7 cases test ed were predominantly infected with wild type HBV strains without any mutat ions in the precore and core promoter regions under the presence of circula ting antibody to hepatitis B e antigen, Interestingly, a predominant clone detected in one donor showed a 63-nucleotide deletion in the precore region including an encapsidation signal sequence. Our findings indicate that the majority of healthy individuals positive for anti-HBc, which had been assu med to denote a past history of transient HBV infection, were latently infe cted with the episomal form of HBV accompanied by ongoing viral replication and few nucleotide mutations in the precore and core regions.