Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae

Citation
R. Nielsen et al., Evaluation of an indirect enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to the Apx toxins of Actinobacillus pleuropneumoniae, VET MICROB, 71(1-2), 2000, pp. 81-87
Citations number
13
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health",Microbiology
Journal title
VETERINARY MICROBIOLOGY
ISSN journal
0378-1135 → ACNP
Volume
71
Issue
1-2
Year of publication
2000
Pages
81 - 87
Database
ISI
SICI code
0378-1135(200001)71:1-2<81:EOAIEI>2.0.ZU;2-0
Abstract
The reference strains of the 12 serotypes of Actinobacillus pleuropneumonia e express one or two of three different RTX exotoxins designated Apr I, Apr II and Apr III. The toxins are important virulence factors. In the present study, ELISAs with purified Apr I, Apr II and Apr III, respectively, as an tigen were evaluated as candidates for serological diagnosis of Actinobacil lus pleuropneumoniae infection in pigs, The pigs were inoculated with bioty pe 1, serotypes 1-12, and biotype 2, serotype 14, respectively. A strong hu moral antibody response was seen to all the three antigens in most pigs irr espective of the serotype used for inoculation. However, titers to the exot oxins secreted by the serotype used for inoculation were generally highest. The results show that toxin proteins of Actinobacillus pleuropneumoniae li ne are antigenically related and that a correlation between serotype and se cretion of exotoxin is not revealed serologically in the ELISA test. (C) 20 00 Elsevier Science B.V. All rights reserved.