Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris

Citation
Pm. Cupit et al., Cloning and expression of single chain antibody fragments in Escherichia coli and Pichia pastoris, LETT APPL M, 29(5), 1999, pp. 273-277
Citations number
19
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
LETTERS IN APPLIED MICROBIOLOGY
ISSN journal
0266-8254 → ACNP
Volume
29
Issue
5
Year of publication
1999
Pages
273 - 277
Database
ISI
SICI code
0266-8254(199911)29:5<273:CAEOSC>2.0.ZU;2-H
Abstract
The potential of recombinant antibody fragments is likely to be fulfilled o nly if they can be produced routinely at high concentrations. We have compa red the ability of Escherichia coli and Pichia pastoris to produce function al recombinant single chain antibody (scAb) fragments. Two scAb fragments w ere expressed, an antihuman type V acid phosphatase (TRAP) and an anti-Pseu domonas aeruginosa lipoprotein I. We report here that, while expression fro m P. pastoris resulted in a significantly increased level of expression of the anti-TRAP scAb compared to E. coli, neither fragment was able to bind i ts target antigen as well as the bacterial product.