Tumor necrosis factor alpha and human Schwann cells: Signalling and phenotype modulation without cell death

Citation
B. Bonetti et al., Tumor necrosis factor alpha and human Schwann cells: Signalling and phenotype modulation without cell death, J NE EXP NE, 59(1), 2000, pp. 74-84
Citations number
42
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
ISSN journal
0022-3069 → ACNP
Volume
59
Issue
1
Year of publication
2000
Pages
74 - 84
Database
ISI
SICI code
0022-3069(200001)59:1<74:TNFAAH>2.0.ZU;2-7
Abstract
The aim of the study was to evaluate the biological response of human Schwa nn cells (SC) to turner necrosis factor alpha (TNF alpha) in vitro and to t he inflammatory milieu of chronic inflammatory demyelinating polyradiculone uritis (CIDP). By immunocytochemical and functional assays, we found that S C expressed TNF receptors and that TNF alpha promoted in SC cultures transi ent activation of transcription factors NF kappa B and c-jun in the absence of apoptosis. In addition, TNF alpha significantly increased the proportio n of non-myelin-forming SC expressing the p75 nerve growth factor receptor. Such phenotypic effect was dose-dependent and partially mediated by NF kap pa B, as assessed by functional blockage with acetylsalicylic acid. We then extended our study to a human disease in which SC are exposed to TNF alpha . Increased signals for NF kappa B, but not c-jun, molecules were observed by immunohistochemistry on SC nuclei in nerve biopsies from patients with C IDP, as compared with controls. Irrespective of the presence of nerve infla mmation, SC showed no evidence of apoptosis. Taken together, our results su ggested that SC are potential targets of TNF alpha and that this cytokine e xerted no cytotoxic effects either in vivo or in vitro. Rather, TNF alpha m ay influence the fate of SC by activating transcriptional pathways and modu lating their phenotype.