Determination of diclofenac sodium by capillary zone electrophoresis with electrochemical detection

Authors
Citation
Wr. Jin et J. Zhang, Determination of diclofenac sodium by capillary zone electrophoresis with electrochemical detection, J CHROMAT A, 868(1), 2000, pp. 101-107
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A
ISSN journal
0021-9673 → ACNP
Volume
868
Issue
1
Year of publication
2000
Pages
101 - 107
Database
ISI
SICI code
0021-9673(20000128)868:1<101:DODSBC>2.0.ZU;2-Q
Abstract
Capillary zone electrophoresis was employed for the determination of diclof enac sodium using an end-column amperometric detection with a carbon fiber microelectrode, at a constant potential of 0.83 V vs. saturated calomel ele ctrode. The optimum conditions of separation and detection are 4.90.10(-3) mol/l Na2HPO4-3.10.10(-3) mol/l NaH2PO4 (pH 7.0) for the buffer solution, 1 0 kV for the separation voltage, 5 kV and 10 s for the injection voltage an d the injection time, respectively. The limit of detection is 2.5.10(-6) mo l/l or 5.2 fmol (S/N=2). The relative standard deviation is 0.8% for the mi gration time and 4.7% for the electrophoretic peak current. The method was applied to the determination of diclofenac sodium in human urine. (C) 2000 Elsevier Science B.V. All rights reserved.