Streptavidin arrays as supramolecular architectures in surface-plasmon optical sensor formats

Citation
W. Knoll et al., Streptavidin arrays as supramolecular architectures in surface-plasmon optical sensor formats, COLL SURF A, 161(1), 2000, pp. 115-137
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Physical Chemistry/Chemical Physics
Journal title
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
ISSN journal
0927-7757 → ACNP
Volume
161
Issue
1
Year of publication
2000
Pages
115 - 137
Database
ISI
SICI code
0927-7757(20000115)161:1<115:SAASAI>2.0.ZU;2-Q
Abstract
We describe a series of interfacial biomolecular architectures employed for studies aimed at elucidating the structural and dynamical factors governin g biorecognition and binding reactions between surface-attached biotin-deri vatives and analogues and streptavidin from solution. We compare binding ma trices based on mixed lipid monolayers transferred to a solid support from the water/air-interface and surface coatings based on binary mixed self-ass embled monolayers. The analytical techniques used to monitor the various la yers and the binding events are based on surface-plasmon spectroscopy and m icroscopy, in some cases in combination with electrochemical methods contro lling, in particular, the surface potential of electrode surfaces in contac t with aqueous electrolyte solutions. Finally, an example is given for the sensitivity enhancement obtainable by combining the field-enhancement of su rface-plasmon resonances with fluorescence detection schemes. (C) 2000 Else vier Science B.V. All rights reserved.