cADP-ribose potentiates cytosolic Ca2+ elevation and Ca2+ entry via L-typevoltage-activated Ca2+ channels in NG108-15 neuronal cells

Citation
M. Hashii et al., cADP-ribose potentiates cytosolic Ca2+ elevation and Ca2+ entry via L-typevoltage-activated Ca2+ channels in NG108-15 neuronal cells, BIOCHEM J, 345, 2000, pp. 207-215
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL JOURNAL
ISSN journal
0264-6021 → ACNP
Volume
345
Year of publication
2000
Part
2
Pages
207 - 215
Database
ISI
SICI code
0264-6021(20000115)345:<207:CPCCEA>2.0.ZU;2-#
Abstract
The effects of cADP-ribose (cADPR), a metabolite of beta-NAD(+), on the ele vation of cytoplasmic free Ca2+ concentration ([Ca2+](i)) and Ca2+ flux thr ough voltage-activated Ca2+ channels (VACCs) were studied in NG108-15 neuro blastoma x glioma hybrid cells. NG108-15 cells were pre-loaded with fura-2 and whole-cell patch-clamped. Application of cADPR through patch pipettes d id not by itself trigger any [Ca2+](i) rise at the resting membrane potenti al. A rise in [Ca2+](i) was evoked upon sustained membrane depolarization, and was significantly larger in cADPR-infused cells than in non-infused cel ls. This potentiation in the [Ca2+](i) elevation was reproduced by infusion of beta-NAD(+), and was blocked by s-bromo-cADPR and antagonized by extern al application of ryanodine or by pretreatment of cells with FK506. Nicotin amide inhibited beta-NAD(+)-induced, but not cADPR-elicited, potentiation. [Ca2+](i) increases or Ca2+ influx, measured by Mn2+ quenching, elicited by the same protocol of depolarization was blocked completely by nifedipine b ut not by omega-conotoxin. Ca2+ influx in cADPR- or beta-NAD(+)-infused cel ls was steeper and greater than that in control cells, and was inhibited pa rtly by ryanodine. In contrast, ryanodine accelerated Ca2+ influx in non-in fused cells. These results show that cADPR amplifies both depolarization-in duced [Ca2+](i) increase and Ca2+ influx through L-type VACCs. These result s suggest that cADPR functions on ryanodine receptors as a direct agonist a nd also interacts with L-type VACCs as an indirect agonist, i.e. via a retr ograde signal.