Constitutive and cytokine-regulated expression of presenilin-1 and presenilin-2 genes in human neural cell lines

Citation
Ji. Satoh et Y. Kuroda, Constitutive and cytokine-regulated expression of presenilin-1 and presenilin-2 genes in human neural cell lines, NEUROP AP N, 25(6), 1999, pp. 492-503
Citations number
60
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
ISSN journal
0305-1846 → ACNP
Volume
25
Issue
6
Year of publication
1999
Pages
492 - 503
Database
ISI
SICI code
0305-1846(199912)25:6<492:CACEOP>2.0.ZU;2-T
Abstract
To investigate the role of pleiotropic neuronal and glial cytokines in the regulation of presenilin (PS) gene expression in human neural cells, both p resenilin-1 (PS1) and presenilin-2 (PS2) mRNA levels were analysed by North ern blotting in SK-N-SH neuroblastoma, IMR-32 neuroblastoma, NTera2 teratoc arcinoma-derived differentiated neurones (NTera2-N) and U-373MG astrocytoma cells following exposure to proinflammatory cytokines (TNF-alpha, IFN-gamm a, or IL-1 beta), anti-inflammatory cytokines (IL-10 or TGF-beta 1), dibuty ryl cyclic AMP or phorbol 12-myristate 13-acetate (PMA). The constitutive e xpression of PS1 (3.0 kb) and PS2 (2.3 kb) mRNA was identified in all these cell lines, in which PS1 mRNA levels were unaltered following treatment wi th any cytokines and factors examined. By contrast, PS2 mRNA expression was upregulated substantially in SK-N-SH cells by exposure to TNF-alpha and in U-373MG cells by treatment with IFN-gamma, whereas it was downregulated in both NTera2-N and U-373 MG cells following exposure to IL-1 beta or PMA. T he levels of PS2 mRNA remained unchanged in IMR-32 cells after these treatm ents. These results indicate that PS1 and PS2 genes are expressed constitut ively in a panel of human neural cell lines where PS2 mRNA expression is af fected by a distinct set of cytokines via cell type-specific mechanisms tha t do not alter PS1 mRNA levels, suggesting the existence of separated regul atory systems controlling the expression of PS1 and PS2 genes in human neur al cells.