Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli

Citation
Yj. Song et al., Distinctness of spore and vegetative cellular fatty acid profiles of some aerobic endospore-forming bacilli, J MICROB M, 39(3), 2000, pp. 225-241
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
JOURNAL OF MICROBIOLOGICAL METHODS
ISSN journal
0167-7012 → ACNP
Volume
39
Issue
3
Year of publication
2000
Pages
225 - 241
Database
ISI
SICI code
0167-7012(200002)39:3<225:DOSAVC>2.0.ZU;2-G
Abstract
A gas chromatographic analysis method was employed to determine the cellula r fatty acid (CFA) profiles of spores and vegetative cells of some aerobic endospore-forming bacilli. The harvests of experimental strains were proces sed to obtain pure spores and acquire whole cell fatty acid methyl esters f or the subsequent gas chromatographic analysis, and the corresponding veget ative cells were set as control. Evaluation of reproducibility of spore CFA components revealed that, provided under standardized experimental procedu re, spore CFA composition was stable enough for research purposes. Fatty ac ids recovered in spores in greater quantities were saturated branched-chain acids containing 15 and 17 carbon atoms, similar to the vegetative cells. Commonly, the proportions of saturated branched-chain acids in spores were greater than in vegetative cells. The dendrograms obtained by cluster analy sis provided some meaningful taxonomic information of the experimental stra ins. The fatty acids analysis of spores seems to be a promising supplementa ry tool for the chemotaxonomic research of aerobic endospore-forming bacill i. (C) 2000 Elsevier Science B.V. All rights reserved.