Induction of Fas and Fas-ligand expression in plasmacytoma cells by a cytotoxic factor secreted by marine macrophages

Authors
Citation
Cy. Chu et J. Tseng, Induction of Fas and Fas-ligand expression in plasmacytoma cells by a cytotoxic factor secreted by marine macrophages, J BIOMED SC, 7(1), 2000, pp. 58-63
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
JOURNAL OF BIOMEDICAL SCIENCE
ISSN journal
1021-7770 → ACNP
Volume
7
Issue
1
Year of publication
2000
Pages
58 - 63
Database
ISI
SICI code
1021-7770(200001/02)7:1<58:IOFAFE>2.0.ZU;2-Q
Abstract
Induction of tumoricidal activity is one of the major functions of activate d macrophages. Our previous study demonstrated that P388D1 murine macrophag e-like cells secreted a plasmacytoma cytotoxic factor (PCF) that selectivel y killed certain tumor cell lines including MOPC-315 plasmacytoma in vitro. Our subsequent studies demonstrated that PCF killed MOPC-315 cells by indu ction of apoptosis, In this report, the involvement of Fas and Fas ligand ( FasL) in PCF-induced apoptosis was investigated. Results suggest that expre ssion of Fas mRNA time-dependently increased in PCF-treated cells and reach ed an optimal level after 36 h of treatment. The augmented effect of PCF on Fas mRNA expression was significantly reduced by the addition of CB7.C2, a n anti-PCF monoclonal antibody. The expression of Fast mRNA was also induce d by PCF-and reached an optimal level at 24 h, but sharply decreased after 36 h of treatment, Caspase-3 is one of the proteolytic enzymes that can be activated by the Fas-FasL interaction, In our studies, the enzymatic activi ty of caspase-3 was significantly induced by PCF after 6 h of treatment and reached an optimal level at 12 h, The augmented effect of PCF on caspase a ctivity was significantly reduced by the addition of CB7.C2 and the caspase -3-specific inhibitor, DEVD-fmk. Therefore, PCF-treated plasmacytoma cells might undergo apoptosis via interaction between Fas and Fast. Copyright (C) 2000 National Science Council. ROC and S. Karger AG. Basel.