Production of the antibiotic gallidermin by Staphylococcus gallinarum - development of a scale-up procedure

Citation
M. Kempf et al., Production of the antibiotic gallidermin by Staphylococcus gallinarum - development of a scale-up procedure, BIOTECH LET, 22(2), 2000, pp. 123-128
Citations number
11
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
BIOTECHNOLOGY LETTERS
ISSN journal
0141-5492 → ACNP
Volume
22
Issue
2
Year of publication
2000
Pages
123 - 128
Database
ISI
SICI code
0141-5492(200001)22:2<123:POTAGB>2.0.ZU;2-#
Abstract
A scale-up strategy into 200 l pilot-scale for the production of the antibi otic gallidermin by Staphylococcus gallinarum Tu 3928 was developed. Large- scale fermentations were simulated by consecutive liquid cultures of smalle r scale. Afterwards, optimised cultivation conditions were transferred to p ilot-scale. Best results were achieved by addition of Maltose during the la te production phase leading to a final concentration of 330 mg gallidermin per litre. Compared to the concentrations found in a non-pulsed pilot-scale fermentations this is an increase of 20-30%.