Metabolic engineering of p-hydroxybenzylglucosinolate in Arabidopsis by expression of the cyanogenic CYP79A1 from Sorghum bicolor

Citation
S. Bak et al., Metabolic engineering of p-hydroxybenzylglucosinolate in Arabidopsis by expression of the cyanogenic CYP79A1 from Sorghum bicolor, PLANT J, 20(6), 1999, pp. 663-671
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
20
Issue
6
Year of publication
1999
Pages
663 - 671
Database
ISI
SICI code
0960-7412(199912)20:6<663:MEOPIA>2.0.ZU;2-E
Abstract
Glucosinolates are natural products in cruciferous plants, including Arabid opsis thaliana. CYP79A1 is the cytochrome P450 catalysing the conversion of tyrosine to p-hydroxyphenylacetaldoxime in the biosynthesis of the cyanoge nic glucoside dhurrin in sorghum. Both glucosinolates and cyanogenic glucos ides have oximes as intermediates. Expression of CYP79A1 in A. thaliana res ults in the production of high levels of the tyrosine-derived glucosinolate p-hydroxybenzylglucosinolate, which is not a natural constituent of A. tha liana. This provides further evidence that the enzymes have low substrate s pecificity with respect to the side chain. The ability of the cyanogenic CY P79A1 to integrate itself into the glucosinolate pathway has important impl ications for an evolutionary relationship between cyanogenic glucosides and glucosinolates, and for the possibility of genetic engineering of novel gl ucosinolates.