Detection of free radicals generated from hydrogen peroxide, gallic acid and haemoprotein chemiluminescence system by electron spin resonance spectroscopy

Citation
M. Kawane et al., Detection of free radicals generated from hydrogen peroxide, gallic acid and haemoprotein chemiluminescence system by electron spin resonance spectroscopy, LUMINESCENC, 14(6), 1999, pp. 321-325
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
LUMINESCENCE
ISSN journal
1522-7235 → ACNP
Volume
14
Issue
6
Year of publication
1999
Pages
321 - 325
Database
ISI
SICI code
1522-7235(199911/12)14:6<321:DOFRGF>2.0.ZU;2-U
Abstract
Low-level chemiluminescence is produced in a hydrogen peroxide (H2O2)/galli c acid/haemoprotein system with single broad peaks around 520 nm, regardles s of the biological role of the haemoprotein. The free haem iron systems (h aemin and haematin systems) gave a higher photon intensity (1.5 x 10(4) and 2.0 x 10(4) cpa) than that of the H2O2/gallic acid/haematoporphyrin system . These results indicated that haem iron plays a significant role in the ph oton emission of haemoprotein systems. A free radical with a g value of 2.0 058 was detected through a direct electron spin resonance (ESR) method. The photon intensity of the H2O2/gallic acid/ haemoprotein system decreased in the order: HRP > cytochrome c > myoglobin > haemoglobin, and this correspo nded to the decrease in radical intensity. These results indicated that the formation of the free radical with a g value of 2.0058 may be the key step for chemiluminescence in the H2O2/gallic acid/haemoprotein system. A quarr el line similar to DMPO-OH adducts and uncomplexed free radicals (g = 2.005 8) was detected using the ESR spin-trapping method in the H2O2/gallic acid/ cytochrome c system. Copyright (C) 1999 John Wiley & Sons, Ltd.