HIV-1 gp120 induces IL-4 and IL-13 release from human Fc epsilon RI+ cellsthrough interaction with the V(H)3 region of IgE

Citation
V. Patella et al., HIV-1 gp120 induces IL-4 and IL-13 release from human Fc epsilon RI+ cellsthrough interaction with the V(H)3 region of IgE, J IMMUNOL, 164(2), 2000, pp. 589-595
Citations number
63
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
0022-1767 → ACNP
Volume
164
Issue
2
Year of publication
2000
Pages
589 - 595
Database
ISI
SICI code
0022-1767(20000115)164:2<589:HGIIAI>2.0.ZU;2-R
Abstract
HIV-1 glycoprotein (gp) 120 from different clades is a potent stimulus for IL-4 and IL-13 release from basophils purified from healthy individuals ser onegative for Abs to HIV-1 and HIV-2, IL-4 mRNA, constitutively present in basophils, was increased after stimulation by gp120 and was inhibited cyclo sporin A and tacrolimus. IL-4 and IL-13 secretion from basophils activated by gp120 was not correlated. There was a correlation between the maximum gp 120- and anti-IgE-induced IL-4 release from basophils. The average t(1/2) g p120-induced IL-4 release was lower than for IL-13 release. Basophils from which IgE had been dissociated by brief exposure to lactic acid no longer r eleased IL-4 in response to gp120 or to anti-IgE, The response to a mAb cro ss-linking the alpha-chain of high-affinity receptor for IgE (Fc epsilon RI ) was unaffected by this treatment. Three human V(H)3(+) monoclonal IgM inh ibited gp120-induced secretion of IL-4 from basophils, In contrast, V(H)6() monoclonal IgM did not inhibit the release of IL-4 induced by gp120, Synt hetic peptides distant from the NH2 and. COOH termini of gp120(MN) inhibite d the activating property of gp120(MN). These results indicate that gp120, which acts as a viral superantigen, interacts with the V(H)3 region of IgE to induce the release of IL-4 and IL-13 from human Fc epsilon RI+ cells.