Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes

Citation
X. Liu et al., Basic fibroblast growth factor up-regulates the expression of vascular endothelial growth factor in primary cultured rat astrocytes, ACT PHAR SI, 21(1), 2000, pp. 19-22
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
ACTA PHARMACOLOGICA SINICA
ISSN journal
0253-9756 → ACNP
Volume
21
Issue
1
Year of publication
2000
Pages
19 - 22
Database
ISI
SICI code
0253-9756(200001)21:1<19:BFGFUT>2.0.ZU;2-P
Abstract
AIM: To examine the effect of recombinant human basic fibroblast growth fac tor(bFGF) on the expression of vascular endothelial growth factor (VEGF) in primary cultured rat astrocytes. METHODS: Semiquantification PCR (SQ-PCR) and immunocytochemistry were used to investigate the effect of bFGF on VEGF mRNA level and protein level, respectively. RESULTS: Treatment with bFGF d ose-dependently increased the VEGF mRNA level in astrocytes. The up-regulat ion of VEGF mRNA induced by bFGF (10 mu g/L) was detected as short as 3-h t reatment. The increase of VEGF mRNA level reached the maximum after 24-h tr eatment with bFGF. The immunocytochemical staining showed that the VEGF pro tein level in astrocytes also increased after the cells were incubated with bFGF. CONCLUSION: bFGF induced a marked time- and concentration-dependent increase in VEGF expression in primary cultured astrocytes, suggesting that the effect of bFGF on angiogenesis in brain may act partly by up-regulatin g VEGF expression in astrocytes.