The effect of melatonin on in vitro fertilization and embryo development in mice

Citation
B. Ishizuka et al., The effect of melatonin on in vitro fertilization and embryo development in mice, J PINEAL R, 28(1), 2000, pp. 48-51
Citations number
32
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Physiology
Journal title
JOURNAL OF PINEAL RESEARCH
ISSN journal
0742-3098 → ACNP
Volume
28
Issue
1
Year of publication
2000
Pages
48 - 51
Database
ISI
SICI code
0742-3098(200001)28:1<48:TEOMOI>2.0.ZU;2-7
Abstract
To examine the effect of melatonin on in vitro fertilization and embryonic development, mouse embryos after insemination in vitro were cultured in a p hysiological medium with or without melatonin. Melatonin increased the fert ilization rate significantly at a concentration between 10(-6) and 10(-4) M (27.6 vs. 43.9 or 40.4%, P < 0.01). Furthermore, a significant increase in the rate of embryos reaching the four-cell stage (16.0 vs. 26.7%, P < 0.01 ), the eight-cell stage (12.1 vs. 25.8 or 23.5%, P < 0.01), and blastulatio n (8.9 vs. 23.5 or 17.5%, P < 0.01) was observed when the embryos were cult ured in a medium containing 10(-8) or 10(-6) M melatonin. These results dem onstrate that melatonin supports fertilization and early embryo development after in vitro fertilization.