Bifurcation of cell migratory and proliferative signaling by the adaptor protein Shc

Citation
Lr. Collins et al., Bifurcation of cell migratory and proliferative signaling by the adaptor protein Shc, J CELL BIOL, 147(7), 1999, pp. 1561-1568
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
147
Issue
7
Year of publication
1999
Pages
1561 - 1568
Database
ISI
SICI code
0021-9525(199912)147:7<1561:BOCMAP>2.0.ZU;2-R
Abstract
Cytokines and extracellular matrix proteins initiate signaling cascades tha t regulate cell migration and proliferation. Evidence is provided that the adaptor protein Shc can differentially regulate these processes. Specifical ly, under growth factor-limiting conditions, Shc stimulates haptotactic cel l migration without affecting anchorage-dependent proliferation, However, w hen growth factors are present, Shc no longer influences cell migration; ra ther, Shc is crucial for DNA synthesis. Mutational analysis of Shc demonstr ates that, while tyrosine phosphorylation is required for both DNA synthesi s and cell migration, the switch in Shc signaling is associated with differ ential use of Shc's phosphotyrosine interacting domains; the PTB domain reg ulates haptotaxis, while the SH2 domain is selectively required for prolife ration.