Molecular dissection of zyxin function reveals its involvement in cell motility

Citation
Be. Drees et al., Molecular dissection of zyxin function reveals its involvement in cell motility, J CELL BIOL, 147(7), 1999, pp. 1549-1559
Citations number
53
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cell & Developmental Biology
Journal title
JOURNAL OF CELL BIOLOGY
ISSN journal
0021-9525 → ACNP
Volume
147
Issue
7
Year of publication
1999
Pages
1549 - 1559
Database
ISI
SICI code
0021-9525(199912)147:7<1549:MDOZFR>2.0.ZU;2-M
Abstract
Spatially controlled actin filament assembly is critical for numerous proce sses, including the vectorial cell migration required for wound healing, ce ll-mediated immunity, and embryogenesis. One protein implicated in the regu lation of actin assembly is zyxin, a protein concentrated at sites where th e fast growing ends of actin filaments are enriched. To evaluate the role o f zyxin in vivo, we developed a specific peptide inhibitor of zyxin functio n that blocks its interaction with alpha-actinin and displaces it from its normal subcellular location. Mislocalization of zyxin perturbs cell migrati on and spreading, and affects the behavior of the cell edge, a structure ma intained by assembly of actin at sites proximal to the plasma membrane. The se results support a role for zyxin in cell motility, and demonstrate that the correct positioning of zyxin within the cell is critical for its physio logical function. Interestingly, the mislocalization of zyxin in the peptid e-injected cells is accompanied by disturbances in the distribution of Ena/ VASP family members, proteins that have a well-established role in promotin g actin assembly. In concert with previous work, our findings suggest that zyxin promotes the spatially restricted assembly of protein complexes neces sary for cell motility.